Catalogue number
PRO-2259
Synonyms
C10orf54, Chromosome 10 Open Reading
Frame 54, V-Domain Ig Suppressor Of T Cell Activation, Stress-Induced Secreted Protein-1, Sisp-1, SISP1, Stress Induced Secreted Protein 1, Death Domain1alpha, DD1alpha, PP2135, B7-H5, VISTA,
B7H5, GI24.
Introduction
Chromosome 10 Open Reading Frame 54, also known as C10ORF54 is part of
the immunoglobulin superfamily. Like a transmembrane molecule, C10ORF54 is
expressed in the bone on embryonic stem cells (ESCs), and on tumor cell surface as well. Accordingly, C10ORF54 supports the differentiation of
ESC and ehhances BMP4 induced signaling in ESC, however C10ORF54 is also down
regulated following to BMP4 exposure.
Description
C10ORF54 Human Recombinant produced in Sf9 Baculovirus
cells is a single, glycosylated polypeptide chain containing 170 amino acids (33-194
a.a) and having a molecular mass of 19.1kDa. (Migrates at 28-40kDa on SDS-PAGE
under reducing conditions). C10ORF54 is fused to an 8 amino acid His-tag at C-terminus
& purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
C10ORF54 protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
FKVATPYSLY
VCPEGQNVTL TCRLLGPVDK GHDVTFYKTW YRSSRGEVQT CSERRPIRNL TFQDLHLHHG GHQAANTSHD
LAQRHGLESA SDHHGNFSIT MRNLTLLDSG LYCCLVVEIR HHHSEHRVHG AMELQVQTGK DAPSNCVVYP
SSSQDSENIT AAVEHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
