Catalogue number
PRO-1275
Synonyms
BTCD, KIN17, KIN, Binding to curved DNA, antigenic determinant of recA protein homolog, DNA/RNA-binding protein KIN17.
Introduction
DNA/RNA-binding protein KIN17 (KIN17) is a nuclear protein which constructs intranuclear foci for the duration of proliferation and is reallocated in the nucleoplasm during the cell cycle. KIN17 is ubiqutiously expressed having the highest levels of expression in the muscle, heart and testis. SV40- altered fibroblasts overexpress KIN17, which interacts with Large T antigen and decreases T-antigen-dependent DNA replication.
Description
KIN Human Recombinant produced in E. coli is a single polypeptide chain containing 416 amino acids (1-393) and having a molecular mass of 47.8 kDa.
KIN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The KIN solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGKSDFL TPKAIANRIK SKGLQKLRWY CQMCQKQCRD
ENGFKCHCMS ESHQRQLLLA SENPQQFMDY FSEEFRNDFL ELLRRRFGTK RVHNNIVYNE YISHREHIHM NATQWETLTD FTKWLGREGL CKVDETPKGW YIQYIDRDPE TIRRQLELEK
KKKQDLDDEE KTAKFIEEQV RRGLEGKEQE VPTFTELSRE NDEEKVTFNL SKGACSSSGA
TSSKSSTLGP SALKTIGSSA SVKRKESSQS STQSKEKKKK KSALDEIMEI EEEKKRTART
DYWLQPEIIV KIITKKLGEK YHKKKAIVKE VIDKYTAVVK MIDSGDKLKL DQTHLETVIP
APGKRILVLN GGYRGNEGTL ESINEKTFSA TIVIETGPLK GRRVEGIQYE DISKLA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
