Catalogue number
PRO-2403
Synonyms
CFB, AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG,
H2-Bf, PBF2, Complement Factor B, B-Factor, Properdin, Properdin Factor B,
C3/C5 Convertase, EC 3.4.21.47, PBF2,Glycine-Rich Beta-Glycoprotein,
Glycine-Rich Beta Glycoprotein, C3 Proaccelerator, C3 Proactivator , EC 3.4.2,
FBI12, H2-Bf.
Introduction
Complement Factor B, also known as CFB, encodes complement factor B which is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Once the alternative pathway is activated it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which connects with C3b to form the alternative pathway C3 convertase. Also, Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
Description
CFB Human Recombinant produced in Sf9 Baculovirus cells is
a single, glycosylated polypeptide chain containing 245 amino acids (26-259a.a.)
and having a molecular mass of 27.3kDa (Molecular size on SDS-PAGE will appear
at approximately 28-40 kDa). CFB is expressed with a 11 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CFB protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADPEFTPWSL
ARPQGSCSLE GVEIKGGSFR LLQEGQALEY VCPSGFYPYP VQTRTCRSTG SWSTLKTQDQ KTVRKAECRA
IHCPRPHDFE NGEYWPRSPY YNVSDEISFH CYDGYTLRGS ANRTCQVNGR WSGQTAICDN GAGYCSNPGI
PIGTRKVGSQ YRLEDSVTYH CSRGLTLRGS QRRTCQEGGS WSGTEPSCQD SFMYDTPQEV AEAFLSSLTE
TIEGVDAEDG HGPGEQQKRH HHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
