Catalogue number
PRO-815
Synonyms
Regulatory Subunit 22, MINOS3, PPP1R22, Mitochondrial
Inner Membrane Organizing System 3, Protein Phosphatase 1, Coiled-Coil-Helix-Coiled-Coil-Helix
Domain-Containing Protein 3 Mitochondrial, Protein Phosphatase 1, Regulatory
Subunit 22.
Introduction
Coiled-coil-helix-coiled-coil-helix
domain containing 3 (CHCHD3), is a protein-coding gene. CHCHD3 is essential for
maintenance of mitochondrial crista integrity and mitochondrial function. . In
addition, CHCHD3 may perform as a scaffolding
protein that stabilizes protein complexes involved in crista architecture and
protein import. CHCHD3 may also act as a transcription factor which binds to
the BAG1 promoter and represses BAG1 transcription. Lung cancer has been
associated with CHCHD3.
Description
CHCHD3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-227 a.a) and having a molecular mass of 28.5kDa.
CHCHD3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
CHCHD3 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGTTST RRVTFEADEN ENITVVKGIR LSENVIDRMK ESSPSGSKSQ
RYSGAYGASV SDEELKRRVA EELALEQAKK ESEDQKRLKQ AKELDRERAA ANEQLTRAIL RERICSEEER
AKAKHLARQL EEKDRVLKKQ DAFYKEQLAR LEERSSEFYR VTTEQYQKAA EEVEAKFKRY ESHPVCADLQ AKILQCYREN THQTLKCSAL
ATQYMHCVNH AKQSMLEKGG
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
