Catalogue number
PRO-2316
Synonyms
Tissue factor, TF, Coagulation factor III, CD142.
Introduction
Tissue factor is well-known as the main cellular initiator of blood coagulation. The Tissue factor gene encodes coagulation factor III which is a cell surface glycoprotein that enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. Following vessel injury, the Tissue Factor and Factor VIIa complex activates the coagulation protease cascade, which leads to fibrin deposition and activation of platelets. The ensuing complex presents a catalytic event, which is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Therefore, Tissue factor has a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Tissue Factor can also be stimulated by the inflammatory mediators interleukin 1 and TNF, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response.
Tissue factor is the only one in the coagulation pathway for which a congenital deficiency has not been described. Certain levels of Tissue Factor are essential for the maintained viability and growth of endothelium and Tissue Factor-expressing tumor cells. Additionally, abnormal Tissue Factor expression inside the vasculature initiates life threatening thrombosis in various diseases, for example sepsis, atherosclerosis, and cancer. Alternative spliced Tissue Factor expression advances tumor growth, and is linked to increased tumor cell proliferation and angiogenesis in pancreatic cancer.
Description
F3 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 232 amino acids (29-251 a.a.) and
having a molecular mass of 26.4kDa (Migrates at 28-40kDa on SDS-PAGE under
reducing conditions).
F3 is expressed with a 6 amino acid His tag at C-Terminus
and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
F3 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
ADPAGIPEKA FNLTWISTDF KTILEWQPKP TNYTYTVQIS DRSRNWKNKC FSTTDTECDL TDEIVKDVTW AYEAKVLSVP RRNSVHGDGD QLVIHGEEPP FTNAPKFLPY RDTNLGQPVI QQFEQDGRKL NVVVKDSLTL VRKNGTFLTL RQVFGKDLGY IITYRKGSST GKKTNITNTN EFSIDVEEGV SYCFFVQAMI FSRKTNQNSP GSSTVCTEQW KSFLGEHHHH HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
