Catalogue number
PRO-701
Synonyms
MRG1, P35SRJ, CIT-ED2, Cbp/p300-interacting transactivator 2, MSG-related protein 1, MRG-1, CITED2.
Introduction
CITED2 is a significant transcriptional cofactor that takes part in multiple organ development. CITED2 is a cAMP-responsive element-binding protein (CBP)/p300 interacting transcriptional modulator and a negative regulator for HIF1A through its competitive binding with HIF1A to CBP/p300. CITED2 is essential for mouse fetal liver hematopoiesis and is necessary for the appropriate formation of the hyaloid vasculature and for lens morphogenesis. CITED2 is a coactivator of HNF4alpha and necessary for liver development. CITED2 is a coactivator of PPAR-alpha and both participate in signaling cascades of hypoxic response and angiogenesis.
Description
CITED2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 278 amino acids (1-270 a.a.) and having a molecular mass of 29.5 kDa.
The CITED2 is fused to an 8 amino acid His Tag at C-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The CITED2 solution contains 20mM Tris pH-8, 1mM DTT ,0.1M NaCl & 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMGPPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC LEHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
