Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192 31-1175-00|产品详情|进口橙子视频旧款采购网

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Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192

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Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192

品牌：RevMAb

货号：31-1175-00

规格：50 ug

货期：电询

应用：

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Product Details
Product Name	Anti-Histone H3 K27M Rabbit Monoclonal Antibody [RM192]
Product Description	Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant
Catalog No.	31-1175-00
Clone Name	RM192
Product Data Sheet	Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192
Specificity	RM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3.
Immunogen	A peptide corresponding to Histone H3 K27M mutant
Application	Western Blot ELISA Immunohistochemistry Immunocytochemistry
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.01 ug/mL – 1 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.005 – 0.1 ug/mL; Immunocytochemistry (ICC/IF): 0.005 – 0.1 ug/mL.
References for Histone H3 K27M Mutant antibody [RM192]	Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533)
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Product Images
	Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Positive for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK
	Immunostaining of brain tumor tissue sectionsusing both EDTA and Citrate pretreatment methods. Clinically validated to be Negative for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK
	Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding wild type (WT) or K27M mutant proteins of Histone H3.3, using 0.01 ug/mL of anti-Histone H3 K27M rabbit monoclonal antibody clone RM192.
	Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K27M mutant, stained with 0.01 ug/mL of anti-Histone H3 K27M rabbit monoclonal antibody clone RM192.