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Product Details
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Product Name
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Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody [RM263]
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Product Description
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Rabbit monoclonal to Histone H3.3 G34W; Histone H3.3 G34W Mutant
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Catalog No.
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31-1145-00
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Clone Name
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RM263
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Product Data Sheet
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Anti-Histone H3.3 G34W Mutant Rabbit Monoclonal Antibody Data Sheet RM263
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Specificity
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RM263 reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3 .
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Immunogen
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A peptide corresponding to Histone H3.3 G34W mutant
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Application
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Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Chromatin IP
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Species Reactivity
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All
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Size
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50 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
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References for Histone H3.3 G34W Mutant antibody [RM263]
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Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742)
Kato, I. et alGiant Cell Tumours of Bone Treated with Denosumab: Histologic, Immunohistochemical, and H3F3A Mutation AnalysesHistopathology 10.1111/his.13448.(2017). IHC-P; Human. IF; Human Read more (PubMed: 29206281)
Luke J. et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant.Histopathology 10.1111/his.13190.(2017). IHC-P; Human. Read more (PubMed: 28211081)
Amary F. et al. H3F3A (Histone 3.3) G34W Immunohistochemistry: A Reliable Marker Defining Benign and Malignant Giant Cell Tumor of Bone.AM J Surg Pathol.10.1097/PAS.0000000000000859. (2017). IHC-P; Human. Read more (PubMed: 28505000)
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Product Images
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Western Blot analysis of cell lysates prepared from
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Immunohistochemical staining of formalin fixed and
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Immunohistochemical staining of Giant Cell Bone
Image courtesy of Adrienne Flanagan, Department
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Immunohistochemical staining of Giant Cell Bone
Image courtesy of Adrienne Flanagan, Department
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