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MitoStep (Mitochondrial Membrane Potencial Assay)

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MitoStep (Mitochondrial Membrane Potencial Assay)

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MitoStep (Mitochondrial Membrane Potencial Assay)

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MitoStep (Mitochondrial Membrane Potencial Assay)

Reference	Size	Price	Quantity	Add to cart
MITO-100T	100 test	190,00 € tax excl.	The minimum purchase order quantity for the product is 1

Reference

Size

Price

Quantity

Add to cart

MITO-100T

100 test

190,00 € tax excl.

Product Description

Tested Applications: Flow Cytometry
Conjugated: DilC1(5)
Species Reactivity: All mammalian
Storage Buffer: DilC1(5), 500 μlof 10μM in DMSO + The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
Regulatory Status: RUO
Storage Instruction: Store in the dark at 2-8 °C

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Membrane potential is generated and maintained by concentration gradients of ions such as sodium, potassium, chloride, and hydrogen. Mitochondrial drives the accumulation in mitochondria of cationic dyes such as cyanines, and the mitochondrial is reduced when energy metabolism is disrupted, notably in apoptosis. Changes in the mitochondrial potential have been described during necrosis, cell cycle and apoptosis. Mitochondrial uptake of dye is a possible source of fluorescence variance. Flow cytometry can be used to estimate membrane potential in eukaryotic cells. Methods using cyanines dyes can detect changes in membrane potential. Immunostep MitoStep uses a cationic dye DilC1(5) (1,1)

Aplication Reference:

Fernández-Moreno M et al. Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines. Ocotber 2016. PlosOne.

Valverde L. et al. New methodology approach to estimate the energetic efficiency of the Mitochondrial Electron Transport Chain in CD4+ T-lymphocytes. Antropo, 25, 1-9.