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HPLC

100 µg in 250 µl of 50 mM NaHCO3 with high CaCl2 (0.01 mM). Lyophilized

with 250 µl of bi-distilled water

 HPLC-purified mouse calretinin (recombinant, 100 µg) produced in Escherichia Coli, after in vitro cDNA synthesis，This recombinant protein has been purified by HPLC and migrates as a single band of approximately 29 kDa

This protein can be used for immuoblots and absorption experiments. For intracellular microinjection experiments we suggest using our other product AgCR10ic or AgCR100ic

After reconstitution, freeze in small aliquots (e.g. 1 µg) and keep at -80°C. Avoid repeated freezing and thawing