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Ca2+-ATPase 3 (human plasma membrane) PMCA3|产品详情|进口橙子视频旧款采购网




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    Ca2+-ATPase 3 (human plasma membrane)
    品牌:Swant
    货号:PMCA3
    规格:PMCA3
    货期:Swant

    品牌:Swant 货号:PMCA3 品名:Ca2+-ATPase 3 (human plasma membrane) 规格:200 µl

    商品详情 参考文献 相关资料

    产品介绍

    品名

     Rabbit anti-Ca2+-ATPase, isoform 3

    货号

     PMCA3

    规格

     200ul

    克隆性 

    polyclonal 

    宿主动物

     Rabbit

    抗体形式

     Antiserum lyophil.

    产品形式

     Lyophilized whole serum (no preservatives).

    复溶

     with 200 µl of bidistilled water

    产品描述

    The antiserum against Ca2+-ATPase, isoform 3, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evalsuated for specificity and potency: a) by Biotin-Avidin labelling of cryostatesections of 4% paraformaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots of brain membranes or primary nerve cell cultures. It can also be used for immunoprecipitation. The serum was inactivated at 50°C for 30 minutes.

    稀释

    Immunohistochemistry : 1: 1'000 - 1: 2'000. 

    Immunoblots: 1: 1'000 - 1: 2'000. 

    For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. 

    We recommend that the optimal dilutions be determined by titration experiments.

    储存

    After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

    参考文献

    1. Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma-membrane Ca2+-pump. A study using specific antibodies. J. Biol. Chem. 19: 12184- 12190. 2. Stauffer T.P., Guerini D., Celio M.R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+-pump isoforms in the rat brain. Brain Res. 748:21-29.

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