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Oct4: octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, also known as POU5F1 (POU class 5 homeobox 1), octamerbinding transcription factor-3 (Oct-3, Otf-3), modulates embryonic stem (ES) cell populations by influencing lineage commitment.Entrez Protein NP_002692.Oct-3/4 sustains stem-cell selfrenewal and differentiation pathways.Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development.Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene.In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells.Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.

Target	OCT4
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	ELISA, WB
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Purified recombinant fragment of Oct4 (aa193-360) expressed in E. coli.
Isotype	IgG1
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	38.5 kDa
Buffer	Ascitic fluid containing 0.03% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.