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The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells.

Target	CD15
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	X-hapten on lacto-N-fucose pentaosyl lll.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG3
Conjugation	Biotin
Specificity	The CD15 antigen is the X-hapten on lacto-N-fucose pentaosyl lll, which is expressed on circulating granulocytes or tissue granulocytes as well as neutrophils and eosinophils. The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	45 kDa
Swiss Prot	P22084
GeneID	2526
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.