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The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Target	AKT1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Tested Applications	FCM
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Human AKT1.
Purification	Purified Rabbit Polyclonal Antibody.
Isotype	IgG
Conjugation	Unconjugated
Specificity	This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-468 amino acids from the C-terminal region of human AKT1.
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Swiss Prot	P31749
NCBI Accession	NP_001014431.1 NP_001014432.1 NP_005154.2
Buffer	PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.