MBP is a major component of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system, although, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs emerge from the long MBP gene which holds three additional exons located upstream of the classic MBP exons. The Golli mRNAs contain three exons unique to Golli-MBP, spliced in-frame to one or more MBP exons. The second family of transcripts contains only MBP exons and produces the well characterized myelin basic proteins. MBP transcription unit is an essential part of the Golli transcription unit and the arrangement is important for the function and/or regulation of these genes.
Description: MBP Recombinant produced in E. coli is a single polypeptide chain containing 220 amino acids (1-197) and having a molecular mass of 23.9kDa.
MBP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Physical Appearance: Sterile Filtered clear solution.
Formulation: The MBP solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Avoid multiple freeze-thaw cycles.