Lyophilised rabbit polyclonal protein A affinity purified antibody

GFP was introduced as a tool for molecular biology over 20 years ago and is thought of as one of those most significant contributions to modern microscopy. Many related fluorescent proteins are subject to oligomerisation and photobleaching and as such, limit the resolution obtainable from their use. mEOS2 is a photoactivatable green to red photoconvertible fluorescent protein derived from Anthozoa. It has recently emerged and is set to make significant contributions to the enhancement of super resolution microscopy for the study of cellular ultra structure and protein trafficking. There are already a number of papers describing its use in the developing field of Photoactivated localisation microscopy (PALM) reversible saturable optical fluorescence transitions (RESOLFT), stochastic optical reconstruction microscopy (STORM) and three-dimensional high-resolution imaging (McKinney et al. 2009). A010-mEOS2 is a protein A affinity purified rabbit polyclonal antibody raised against purified mEOS2 expressed in E.coli and stains mEOS2 fusion proteins expressed in bacteria and mammalian cells in both western blotting and immunofluorescence microscopy applications.