A fully validated, highly specific affinity purified rabbit polyclonal antibody to α and β isoforms of threonine-286 phosphorylated CaMKII

Ca²⁺/calmodulin-dependent kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in translating Ca²⁺- transient signals into cellular responses. Four separate CaMKII genes are expressed in man (α, β, δ, γ) with isoforms within each gene. CaMKII δ and γ are abundant in the heart. CaMKII is activated by Ca²⁺/CaM binding, and becomes activated for prolonged periods of time following autophosphorylation of Thr-286 or Thr-287 (equivalent residues in different isoforms). This antibody recognizes the active, autophosphorylated enzyme (all isoforms) and thus can measure the abundance of active CaMKII. Prolonged activation of CaMKII is associated with a variety of vascular and cardiac diseases including stroke, atrial fibrillation cardiac arrhythmia & sudden death, cardiac apoptosis, and heart failure.

Antibody form: Lyophilised Protein A Affinity Purified Antibody (IgG). Species recognition: rat, human and mouse. Expected to react with various isoforms from chicken, cow, pig, rabbit, orangutan, zebrafish, ferret and Xenopus laevis.