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Immortalized-Rat-Retinal-Precursor-Cells-(R28)

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Immortalized-Rat-Retinal-Precursor-Cells-(R28)

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Immortalized-Rat-Retinal-Precursor-Cells-(R28)

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	Print Version
Biosesafety Level	II
Organism	Postnatal Day 6 Sprague-Dawley Rat
Source Organ	Retinal Tissue
Growth Properties	Adherent
Population Doubling	39.5 hours
Recommended Seeding Density	Recommended split ratio of 1:2 to 1:3
Markers	IRBP, S-antigen
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with retroviruses carrying 12S E1A gene
Description	The Immortalized Rat Retinal Precursor Cell Line (R28) is derived from postnatal day 6 Sprague-Dawley rat retinal tissue immortalized with the 12S E1A gene of adenovirus. Studies have shown that this cell line possesses retinal neurotransmitter receptors that can respond to neurotransmitter stimulation (i.e. dopamine, serotonin, glycine and acetylcholine) and subpopulations are immunoreactive to GluR1, 2 and 3, N-methyl-D-aspartate (NMDA), and γ-aminobutyric acid-a (GABAa). In addition, neurite growth can be promoted by varying the culture medium condition.The heterogeneity of this Immortalized Rat Retinal Precursor Cell Line provides a diversity of cell types in which more closely simulate a retinal explant and offers differentiation potentials useful in studies involving retinal function. Recently a microarray dataset of R28 cells is published describing the presence and absence of 8799 genes and ESTs that may relevant for investigators with an interest in using this cell line for a particular molecular analysis.
Procedure Overview
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Propagation	The base medium for this cell line is DMEM. To make the completed growth medium, add the following components to the base medium: 10% bovine serum (ThermoFisher) and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.To promote neurite growth, culture the cells in DMEM with 10% bovine serum (ThermoFisher) and 250 M pCPT-cyclic AMP.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	1) Western blotting and immunocytochemistry were used to confirm the E1A transgene expression 2) RT-PCR and immunocytochemistry were used to assess the expression of the photoreceptor specific marker S-antigen and interphotoreceptor retinoid binding protein (IRBP)
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.