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Human-Normal-Subcutaneous-White-Preadipocytes

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Human-Normal-Subcutaneous-White-Preadipocytes

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Human-Normal-Subcutaneous-White-Preadipocytes

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Description	Pre-adipocytes are present throughout *** life in adipose tissue and can proliferate and differentiate into mature adipocytes according to the energy balance, contributing to increases in adipose tissue mass. It has also been demonstrated that adipocyte differentiation are involved in many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer. The Human Primary Subcutaneous White Preadipocytes is derived from human subcutaneous or visceral adipose tissue and can proliferate and differentiate into mature adipocytes using abm**'s Adipocyte Differentiation Medium (
Procedure Overview
Biosesafety Level	II
Organism	Homo sapiens
Source Organ	Subcutaneous or Visceral Adipose Tissue
Growth Properties	Adherent
Morphology	Bipolar
Recommended Seeding Density	10,000-15,000 cells/cm²
Applications	For Research Use Only
Disease	Normal
Propagation	The basal medium for this cell line is Prigrow IV medium available in abm, Cat. No. TM004. To make the complete growth medium, add the following components to the basal medium: 10% fetal bovine serum (TM999), 10ng/ml rEGF, 1ug/ml hydrocortisone and 90ug/ml heparin, and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. For differentiation from preadipocytes to adipocytes, use abm's Adipocyte Differentiation Medium (TM005) and follow the differentiation protocol provided below.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.