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Anti-Oct 3/4 Antibody

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Anti-Oct 3/4 Antibody

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Overview	Print Version
Product Name	Anti-Oct 3/4 Antibody
Description	Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus, a critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. Data suggest that Oct3/4 is a master regulator of pluripotency that controls lineage commitment.
Subtype	polyclonal
Application	WB, ELISA
Raised In	rabbit
Immunogen	Reacts with an internal sequence of the human Oct 3/4 protein. Sequence is 84% identical and 94% positive in mouse and rat.
Guarantee	abm guarantees that all our Anti-Oct 3/4 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Properties
Formulation	Liquid
Quantity	100 μg
Clone	Polyclonal
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Concentration	1µg/ul
Purity	Affinity purified
Isotype	IgG
Species Reactivity	human
Specific References
Y052494 has been referenced in 1 publication. Zeineddine, D., Papadimou, E., Chebli, K., Gineste, M., Liu, J., Grey, C., Thurig, S., Behfar, A., Wallace, V. A., Skerjanc, I. S. and Puceat, M. Oct-3/4 dose dependently regulates specification of embryonic stem cells toward a cardiac lineage and early heart development. Dev. Cell 11: 535-546, 2006. Niwa, H., Miyazaki, J. and Smith, A. G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genet. 24: 372-376, 2000. Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H. and Smith, A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95: 379-391, 1998.