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Nanog Adenovirus (Human)

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Nanog Adenovirus (Human)

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	Print Version
Vector	pAdeno
Promoter	CMV
Insert	Nanog
Picture
Organism	Human
Accession #	Q9H9S0
Protein Type	Wild Type
Protein Family	Belongs to the Nanog homeobox family.
Functions	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. The product can be used in combination with other iPSC factor for the induction of iPSC reprogramming.
Cell Types	Expressed in fetal gonads, ovary and testis.
Protein locations	Nucleus
Tag	N-term His
Purification	By Ion-Exchange column.
Quality Control	By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming.
Titer	>1x10⁶pfu/mL
Storage	10 mM Tris-HCl, pH 8.0
Shipping	High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Notes	There are 8 more vials in Box 24 Row E.