Cas9 Expressing RL95-2 Cell line |产品详情|进口橙子视频旧款采购网

您好，欢迎来到橙子视频app安卓下载生物网站！请登录注册

我的订单 | 联系橙子视频app安卓下载 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA91橙子视频、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口橙子视频旧款、Quidel代理、Swant代理、PBL Assay Science代理

网站首页产品中心代理品牌新闻资讯资源中心关于橙子视频app安卓下载

所在位置：首页 > 产品中心 > abmgood > abmgood产品

订购信息

上海橙子视频app安卓下载生物科技公司

Tel:400-968-7988 021-33779008

Cas9 Expressing RL95-2 Cell line

品牌：Abmgood

货号：

规格：

货期：

应用：

咨询客服

立即购买加入购物车

Cas9 Expressing RL95-2 Cell line

商品详情参考文献相关资料

	Print Version
Biosesafety Level	II
Organism	Human
SourceOrgan	Uterus
Growth Properties	Adherent
Morphology	Epithelial
Description	RL95-2 cells stably expressing Cas9
Population Doubling	18-36 hours
Markers	Puromycin
Freeze-Thaw Recovery	1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 1500rpm for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO₂.
Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium with the final concentration: 10% fetal bovine serum (TM999), and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Shipping	Dry Ice
Quality Control	1) Puromycin drug selection 2) Western Blot analysis for Cas9 transgene expression
Can't find what you want? Take advantage of our custom service: WT Control Cell Line Expressing Cas9 for Comparison and Genome-wide CRISPR Knockout cell lines
Disclaimer	1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. Please note that if the gene to be knockout may be essential to cell survival, abm will provide a hemizygous pool as the default deliverable to compensate for any lethal effects (unless otherwise requested).