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Immortalized-Rat-Suprachiasmatic-Nucleus-Cells-(SCN2.2)

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Immortalized-Rat-Suprachiasmatic-Nucleus-Cells-(SCN2.2)

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Immortalized-Rat-Suprachiasmatic-Nucleus-Cells-(SCN2.2)

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	Print Version
Biosesafety Level	II
Organism	Long-Evans Rat, fetal
Source Organ	Brain
Growth Properties	Adherent
Morphology	Composed of round and broad flat polygonal-like appearance
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	NSE, PGP, and MAP-2
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene
Description	Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) is pluripotent and has the appearance of both neuronal and glial morphologies in culture. The cells are able to differentiate into different phenotypes, where neuronal markers are expressed (NSE, PGP, and MAP-2), but glial markers are absent. It is a valuable tool for studies relating to the regulation of the central mammalian pacemaker function, and the development of the suprachiasmatic nucleus cell types.
Procedure Overview
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Propagation	Grow cells in poly-D-lysine or laminin-coated plates. The base medium for this cell line is PriGrow VII, available at abm Cat. No. TM017. To make the completed growth medium, add the following components to the base medium for a final concentration of 10% fetal bovine serum (TM999), 2 µg/ml glucose, 2 mM L-glutamine, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Quality Control	1) Immunofluorescent staining and western blot analysis for E1A; 2) Immunocytochemical screening with cell type-specific antigens to distinguish phenotypes
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evalsuate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical locations. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.