| Overview |
Print Version
|
| Product Name |
Anti-Epidermal Growth Factor Receptor (EGFR) Phospho-Tyr1197 Antibody |
| Description |
EGFR is a transmembrane
glycoprotein that is a member of a family of protein
tyrosine kinases crucial in maintaining a normal balance
in cell growth and development. Growth factor
receptors are involved not only in promoting the
proliferation of normal cells but also in the aberrant
growth of many types of human tumors. For example,
the epidermal growth factor receptor (EGFR) is mutated
and/or overexpressed in many common solid human
squamous cell carcinomas including breast, brain,
bladder, lung, gastric, head & neck, esophagus, cervix,
vulva, ovary, and endometrium. Over-expression of the
EGFR gene occurs in carcinomas with and without gene
amplification. EGFR and erbB-2 are particularly
important in breast cancer because increased
production or activation has been associated with poor
prognosis. EGFR belongs to a family of growth factor
receptors, which also includes ErbB-2/HER-2, ErbB-
3/HER-3 and ErbB-4/HER-4. EGFR can heterodimerize
with each of the members of this family. |
| Subtype |
polyclonal |
| Application |
WB, IHC, ELISA |
| Raised In |
rabbit |
| Specificity |
This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at
the pY1197 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum
was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then
cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human EGFR
pY1197 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated
human EGFR is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with phosphorylated EGFR from
human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown. |
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a
synthetic peptide corresponding to amino acids 1189-1199 of human EGFR protein. |
| Guarantee |
abm guarantees that all our Anti-Epidermal Growth Factor Receptor (EGFR) Phospho-Tyr1197 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Properties |
|
| Formulation |
Liquid |
| Quantity |
100 μg |
| Clone |
Polyclonal |
| Storage |
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Storage Buffer |
PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration |
1µg/ul |
| Purity |
Affinity Purified |
| Isotype |
IgG |
| Species Reactivity |
human |
| Anti-Epidermal Growth Factor Receptor (EGFR) Phospho-Tyr1197 Antibody Images |
|
| Specific References |
|
Y052117 has been referenced in 1 publication.
-
Sartor, C.I. et al., (1997) The Role of EGF Receptor and STAT-3 Activation in Autonomous Proliferation of SUM-102PT Human Breast Cancer
Cells. Cancer Research 57:978.
Scambia G, et al., (1993) Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: Correlation with histopathological
features and receptors for estrogen, progesterone, and the epidermal growth factor. Am J Obstet gynecol 168:71-78.
Henzen-Logmans, S.C., et al., (1993) Occurrence of epidermal growth factor receeptors in benign and malignant ovarian tumors and normal
ovarian tissues: an immunohistochemical study. J Can Res Clin Oncol 188:303-307.
Sibilia, M., A.B. et al., (2000). The EGF Receptor Provides an Essential Survival Signal for SOS-Dependent Skin Tumor Development. Cell
102, 211-220.
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