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A water-soluble dye used for the dye exclusion test for cell viability to distinguish between viable and nonviable cells by seeping into nonviable cells with damaged plasma membranes causing them to appear blue. CAUTION: Do not leave on cells for long periods of time; viable cells may also begin to take up the dye.

Additional Information

Synonyms	Azidine Blue 3B; Benzamine Blue 3B; Benzo Blue 3B; Chlorazol Blue 3B; Diamine Blue 3B; Dianil Blue H3G ; Direct Blue 14; Niagara Blue 3B
Product #	T-2470
CAS #	72-57-1
Chemical Name	Azidine Blue 3B; Benzamine Blue 3B; Benzo Blue 3B; Chlorazol Blue 3B; Diamine Blue 3B; Dianil Blue H3G ; Direct Blue 14; Niagara Blue 3B
Formula	C34 H24 N6 O14 S4 . 4 Na
MW	960.816
Appearance	Liquid
Melting Point	300 °C
Storage Temp	Room Temp
Use	Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability

Additional Information

MDL Number	MFCD00003969
ChemACX	X1005024-1
InChI	InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)/N=N/C3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)/N=N/C5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]