A cationic carbocyanine dye, JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl carbocyanine iodide) dye widely used in apoptosis studies to monitor mitochondrial health. It can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria.
JC-1 dye exhibits potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~529 nm) to red (~590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. The ratio of green to red fluorescence depends only on the membrane potential and not on other factors such as mitochondrial size, shape, and density, which may influence single-component fluorescence signals.
Use of fluorescence ratio detection therefore allows researchers to make comparative measurements of membrane potential and determine the percentage of mitochondria within a population that respond to an applied stimulus.
Additional Information
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Synonyms | 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl carbocyanine iodide |
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Product # | J-2517 |
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CAS # | 3520-43-2 and 47729-63-5 |
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Formula | C25H27Cl4IN4 |
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MW | 652.2 |
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Appearance | Red powder. |
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Purity | ≥98% (NMR) |
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Solubility | May be dissolved in water (50 mg/ml); DMSO (15 mg/ml) |
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Storage Temp | -20°C |
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Additional Information
