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*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker.?G418 disulfate is freely soluble in water. Bacteria and algae require 5 μg/ml or less while animal cells may require 300-500 μg/ml.??It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

Resistance to G-418 is conferred through thenptll?gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells.?G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

?Purity data is for a concentration of 50mg/ml active.

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Additional Information

Additional Information