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hygromycin CAS [31282-04-9], CRISPR-Cas9-Mediated Genome Editing

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hygromycin CAS [31282-04-9], CRISPR-Cas9-Mediated Genome Editing

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CAS:31282-04-9Formula:C20H37N3O13MW:527.52Appearance:Clearandyellowishsolution(HygromycinBinPBSbuffer)Purity:91.8%

商品详情参考文献相关资料

Hygromycin B is an aminoglycoside antibiotic isolated from?Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocations. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

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Additional Information

Product #	H-1012-PBS
CAS #	31282-04-9
Chemical Name	O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Formula	C₂₀H₃₇N₃O₁₃
MW	527.52
Appearance	Clear and yellowish solution (Hygromycin B in PBS buffer)
Purity	91.8%
Solubility	Soluble in Water
Preparation	Working solutions (<2mg>
Storage Temp	Store at -4°C. Protect from light.
Therapeutic Area	Infectious Diseases
Use	Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi

Additional Information

Merck Index	13.4878
MDL Number	MFCD06795479
ChemACX	X1009739-3
InChI	InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILES	CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N