小梁网位于虹膜底部周围，并有一个开口进入巩膜静脉窦，将废弃物质传输至静脉血管。小梁网细胞在房水的流出中起重要作用，且这条路径是眼压依赖的。小梁网细胞的损伤和死亡与开角青光眼的发病有关。小梁网细胞进一步的表达神经递质和神经肽受体，受极低浓度的血管活性多肽和生长因子调节，使这些细胞对小梁网的多层次渗透。体外培养TMC是研究小梁网调节功能的良好模型，并对青光眼治疗技术的开发非常有帮助。
Sciencel的原代冻存，冰冻运输。每管细胞密度*过5×10^5/ml。细胞特性经α-平滑肌肌动蛋白和纤维粘连蛋白免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell实验室提供的实验条件，可保证此细胞15倍增增殖。
推荐培养基：小梁网细胞培养基 (TMCM, Cat. #6591)
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货号
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6590
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产地
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美国
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缩写
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HTMC
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规格
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5 x 10^5 /1ml
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用途
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科研
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储存
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液氮
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运输
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干冰
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1.) Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonz?lez A, Panjwani N. (2012) "Galectin-8 promotes cytoskeletal rearrangement in trabecular meshwork cells through activation of Rho signaling." PLoS One. 7: e44400.
2.) Famili A, Ammar DA, Kahook MY. (2013) "Ethyl pyruvate treatment mitigates oxidative stress damage in cultured trabecular meshwork cells." Mol Vis. 19: 1304-9.
3.) Sacc? SC, La Maestra S, Micale RT, Larghero P, Travaini G, Baluce B, Izzotti A. (2011) "Ability of dorzolamide hydrochloride and timolol maleate to target mitochondria in glaucoma therapy." Arch Ophthalmol. 129: 48-55.
4.) Miyamoto N, Izumi H, Miyamoto R, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Quercetin induces the expression of peroxiredoxins 3 and 5 via the Nrf2/NRF1 transcription pathway." Invest Ophthalmol Vis Sci. 52: 1055-63.
5.) Hudson BD, H?bert TE, Kelly ME. (2010) "Physical and functional interaction between CB1 cannabinoid receptors and beta2-adrenoceptors." Br J Pharmacol. 160: 627-42.
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Question:
Hello. I would like to know how long the human trabecular meshwaork cells survive in culture? For example, 20 passages or 15 doublings? Thank you
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??Answers:
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Dear?Mijin,
According to the?product sheet, we guarantee 15 population doublings for this cell. Thanks!?
