血管平滑肌细胞是许多动脉疾病的主要靶细胞。血管平滑肌细胞的加速生长潜能是血管疾病进展的关键因素。*新研究表明血管平滑肌细胞表达的血管内皮细胞表达细胞粘附因子ICAM-1 和 VCAM-1，研究表明这些因子能促进血管内皮细胞增殖，分化和血管壁的炎症反应，并且与血管疾病的进展及稳定有关。人血管平滑肌细胞的体外培养是血管研究中的重要模型，并将对血管疾病的药理学和治疗研究提供大量信息。
ScienCell实验室的HBVSMC分离自人大脑组织，原代冻存，冰冻运输。每管细胞密度*过5×105/ml。细胞经α-smooth muscle actin和desmin免疫荧光鉴定。本细胞经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。如采用ScienCell 实验室特制的培养基，可保证此细胞达到15次或以上的倍增。
推荐培养基：平滑肌细胞培养基 (SMCM, Cat. #1101)
1.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) 'Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits.'
2.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) 'Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model.'
3.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) "Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits." Biomed res int. 2014: 836397.
4.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) "Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model." PloS one. 9: e84129.
5.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box." BMC Neurosci. 14: 18.
6.) Meng H, Zhang X, Lee SJ, Wang MM. (2013) "Von Willebrand Factor Inhibits Mature Smooth Muscle Gene Expression through Impairment of Notch Signaling." PLoS One. 8: e75808.
7.) Miners JS, Kehoe P, Love S. (2011). "Neprilysin protects against cerebral amyloid angiopathy and Abeta-induced degeneration of cerebrovascular smooth muscle cells."Brain Patholology. 21: 594-605
8.) Fossati S, Cam J, Meyerson J, Mezhericher E, Romero IA, Couraud PO, Weksler BB, Ghiso J, Rostagno A. (2010) "Differential activation of mitochondrial apoptotic pathways by vasculotropic amyloid-beta variants in cells composing the cerebral vessel walls." FASEB J. 24: 229-41.
9.) Lefterov I, Fitz NF, Cronican AA, Fogg A, Lefterov P, Kodali R, Wetzel R, Koldamova R. (2010) "Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice." J Biol Chem 285: 36945-57.
10.) Kanekiyo T, Bu G. (2009) "Receptor-associated protein interacts with amyloid-beta peptide and promotes its cellular uptake." J Biol Chem. 284: 33352-59.
11.) Wang J, Tuo H, Wang R, Li W, Feng Z, Chen J, Wang D. (2008) "The expression of human brain vascular smooth muscle cell AT receptor after the UL83 gene of HCMV inhibition by small interfering RNAs." Neurol Res. 30: 903-09.
