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Mouse Left/Right Determination Factor 2 (LEFTY2) ELISA Kit EKU05584|产品详情|进口橙子视频旧款采购网




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    Mouse Left/Right Determination Factor 2 (LEFTY2) ELISA Kit
    品牌:
    货号:EKU05584
    规格:96T
    货期:

    Mouse Left/Right Determination Factor 2 (LEFTY2) ELISA Kit

    商品详情 参考文献 相关资料

    Product Description

    Species Reactivity Mus musculus (Mouse)
    Format 96 well plate
    Detection Range 15.62-1000 pg/mL
    Sensitivity 5.5 pg/mL
    Sample Type Serum, plasma and other biological fluids.
    Assay Time 3 hours
    UniProt P57785
    Gene ID 320202
    Gene Name Left/Right Determination Factor 2
    Gene Aliases TGFB4; EBAF; LEFTA; LEFTYA; Endometrial Bleeding Associated Factor; Left-right determination factor A; Transforming growth factor beta-4
    Application ELISA
    Assay Type Sandwich
    Detection Method Colorimetric
    Shipping Condition Blue ice
    Storage Instructions -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Precaution Of Use The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Expiry Date 8 months
    Specificity This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 2 (LEFTY2). No significant cross-reactivity or interference between Left/Right Determination Factor 2 (LEFTY2) and analogues was observed.
    Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 2 (LEFTY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 2 (LEFTY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 2 (LEFTY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 2 (LEFTY2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low,
    Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Restrictions For research use only. Not for diagnostic procedures.
    Quality Systems The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
    Research Area Neuro science;
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