Rabbit Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit EKU04864|产品详情|进口橙子视频旧款采购网

您好，欢迎来到橙子视频app安卓下载生物网站！请登录注册

我的订单 | 联系橙子视频app安卓下载 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA91橙子视频、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口橙子视频旧款、Quidel代理、Swant代理、PBL Assay Science代理

网站首页产品中心代理品牌新闻资讯资源中心关于橙子视频app安卓下载

所在位置：首页 > 产品中心 > Biomatik > Biomatik产品

Rabbit Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

订购信息

上海橙子视频app安卓下载生物科技公司

Tel:400-968-7988 021-33779008

Rabbit Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

品牌：

货号：EKU04864

规格：96T

货期：

应用：

咨询客服

立即购买加入购物车

Rabbit Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

商品详情参考文献相关资料

Species Reactivity	Oryctolagus cuniculus (Rabbit)
Format	96 well plate
Detection Range	0.156-10 ng/mL
Sensitivity	0.054 ng/mL
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Assay Time	3 hours
Gene Name	Hypoxia Inducible Factor 1 Alpha
Gene Aliases	HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, m
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Tumor immunity;Cardiovascular biology;Reproductive science;