Description
This product is specially designed for loading DNA samples that contains high amount of proteins that may form complexes with DNA during gel electrophoresis. This product is suitable for use in prevention of band-shift (due to protein binding) or annealing of DNA during both agarose and polyacrylamide gel electrophoresis. The 6X Loading Dye with SDS contains 2 dyes; bromophenol blue and xylene cyanol FF to track DNA migration during electrophoresis.
Storage
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Store at 4°C for 1 week
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Store at -20°C for long storage
Application
Recommended for electrophoretic DNA sample after digestion with restriction endonuclease, ligation or diphosphorylation reactions.
Usage Recommendation
Add 5 volume of DNA sample to 1 volume of 6X Loading dye with SDS and mix well heat at 65°C for 10 minutes and chill on ice for 3 minutes prior to loading.
Ordering Information
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Catalog No
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Description
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Pack Size
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NM0416
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6X Loading Dye with SDS
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5 x 1 ml
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Download
Manual
6X Loading Dye with SDS
Publication
This Product Has Been Used In:
Chong, S.Y., Rao, P.V. and Soon, Jan Mei (2017) Chong, S.Y., Rao, P.V. and Soon, Jan Mei International Food Research Journal, 24 (4). pp. 18111818
Kepel, B., & Fatimawali, D. (2015) Analysis of 16SrNA Gene and Mercury Reduction Ability of Mercury Resistant Bacteria Isolated from Feces of Patient with Mercury Amalgam at Puskesmas Bahu in Manado. Jurnal Kedokteran Yarsi23(1), p.45-55.
