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 Anti-Exendin-4 ABS 012-35 ABS 012-35|产品详情|进口橙子视频旧款采购网




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    Anti-Exendin-4 ABS 012-35
    品牌：
    货号：ABS 012-35
    规格：200 µg
    货期：

    Anti-Exendin-4
    商品详情 参考文献 相关资料
    Cat. No.
    ABS 012-35
    Product name
    Anti-Exendin-4
    Description

    Mouse monoclonal antibody

    Specificity

    Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.

    Immunogen

    Synthetic exendin-4 

    Application
    ELISA
    Species reactivity (positive)
    Human
    Species reactivity (negative)
    Not determined
    Subclass

    IgG1/k

    Gene ID
    2740
    Clone number
    35
    Epitope

    The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39)amide.

    Content

    Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

    Purification
    Protein-A purified
    Form
    Liquid
    Solvent
    0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
    Storage
    4-8ºC without exposure to light. No precautions necessary during handling.
    Target

    Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Heloderma suspectum. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion.

    Application 1
    ELISA
    ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.
    ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay (1).
    By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. ABS 033-10 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.

    1. Murphy RE, Kinhikar AG, Shields MJ, Rosario JD, Preston R, Levin N, Ward GH (2010) Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. Journal of Pharmaceutical and Biomedical Analysis 53:221-227.

    20547023
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