Description
The ViPrimePLUS Salmonella enterica qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Salmonella enterica genomes in clinical samples (e.g. faeces), food or water samples.
Salmonella enterica is a Gram negative bacterium that comprises of more than 2500 serovars, classified based on their types of surface antigens, lipopolysaccharide (O antigen) and flagellar structures (H antigen). However, only a few showed substantial relevance to human. Salmonella infection causes different illnesses in humans depending on their unique pathogeneses. S. enterica serovar Typhi causes vomiting, diarrhoea and followed by typhoid fever, which may lead to intestinal haemorrhage and organ inflammation. S. enterica serovar Typhimurium and Enteritidis cause gastroenteritis, which includes vomiting, diarrhoea and abdominal cramps. These symptoms are normally self-limiting, with only mortality risk on people with weakened immune system and infants without proper treatment. Subtypes that are of veterinary relevance are Gallinarum (chicken), Choleraesuis (swine), Dublin (cattle) and Marina (reptiles).
ViPrimePLUS Salmonella enterica qPCR Kit is intended for research use only.
Kit Component
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S. enterica Primers and Probe Mix (S. enterica PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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• Clinical samples (e.g. faeces)
• Food samples
• Water samples
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Gene Target
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Invasine A (InvA) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2050
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ViPrimePLUS Salmonella enterica qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Salmonella enterica qPCR Kit
