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Concentration
20-50u/μl
5'…R↓ AATTY…3' 
3'…YTTAA↑R…5'

Reaction Conditions 
1X Buffer V4 
10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 20-fold overdigestion with AcsI, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AcsI for 16 hours at 50°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
AcsI {ApoI}