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Concentration
5-10u/μl
5'…G↓GYRCC…3' 
3'…CCATG↑G…5'

Reaction Conditions 
1X Buffer AccBII 
10mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with AccB1I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccB1I for 16 hours at 37°C.
Supplied with 10X Buffer AccB1I, 10X Buffer UB and Viva Buffer A (Diluent) 
*High enzyme concentration may result in Star Activity

Ordering Information

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Manual
AccB1 I {HgiCI}