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Concentration
10-20u/μl 
5'…GCTAG↓C…3'
3'…C↑GATCG…5'

Reaction Conditions 
1X Buffer V2
10mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl₂, 50mM NaCl and 100µg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg /ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with BmtI, about 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BmtI 16 hours at 37°C. 
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
BmtI {NheI*}