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Concentration
10-30u/μl 
5'…GGN↓NCC…3' 
3'…CCN↑NGG…5'

Reaction Conditions 
1X Buffer V5 
30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with Bmi I, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bmi I for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
BmiI {NlaIV}