Concentration
5-10u/μl
5'…GTT↓AAC…3'
3'…CAA↑TTG…5'
Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with HpaI, more than 60% of the DNA fragments can be ligated and recut.
Overdigestion Assay
Star Activity is observed at greater than 5-fold over digestion of 1μg substrate with HpaI.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1280
|
HpaI
|
200u
|
Downloads
Manual
HpaI
