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Description
AtMax Taq DNA Polymerase is a mixture of thermostable Taq DNA Polymerase, proofreading Pfu DNA Polymerase, anti-Taq DNA Polymerase antibodies, reversible inhibitors and enhancers for automatic "Hot Start" amplification. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher amplification fidelity than possible with unmodified Taq DNA Polymerase. Recommended for use in amplification to obtain DNA products up to 20kb with stringent amplification specificity, sensitivity, fidelity and yield.

Features

• Ultra pure recombinant protein is reversibly complexed with an anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures
• Excellent for multiplex amplification as it exhibits wider tolerance for Mg²+ and salt concentrations.
• Improves amplification results with critical templates, such as those containing GC-rich regions, palindromes or multiple repeats.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.

Unit Definition 
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl₂ , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ml BSA in a final volume of 50μl.

Supplied With

• 10X ViBuffer A (without MgCl₂) 
  500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP.
• 10X ViBuffer S 
  160mM (NH₄)₂SO₄, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl₂ and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
• 50mM MgCl₂

Quality Control 
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
20mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.

Ordering Information

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Manual

AtMax Taq DNA Polymerase

Publication
This Product Has Been Used In:
Lipka, A., Panasiewicz, G.,Majewska, M., Bieniek-Kobuszewska, M., Saveljev, A.P., Pankratovm, A.P., Szafranska, B. (2016) )Identification of the pregnancy-associated glycoprotein family (PAGs) and some aspects of placenta development in the European moose (Alces alces L.). Theriogenology. 86.Pp2119-2135.