Description
The ViPrimePLUS Enterocytozoon bieneusi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Enterocytozoon bieneusi genomes in stool samples.
Enterocytozoon bieneusi is a microsporidial pathogen for both humans and animals which causes diarrhea, particularly in immunocompromised patients such as organ transplant recipients, children, and elderly. Hepatobiliary and pulmonary tract infection are also observed. The E. bieneusi spores are hardy and can survive under harsh environment condition. Thus, aside from zoonotic transmission, waterborne transmission is possible, too as the microspores have been detected in ground, surface, ditch, and crop irrigation water sources. More than 90 genotypes have been identified in a diversity of hosts via internal transcribed sequence. A molecular epidemiology study showed that there are 34 human-exclusive genotypes, while another 11 genotypes are zoonotic and non-host specific as they were found in both human and animals.
ViPrimePLUS Enterocytozoon bieneusi qPCR Kit is intended for research use only.
Kit Component
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E. bieneusi Primers and Probe Mix (E. bieneusi PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. stool)
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Gene Target
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Internal transcribed sequence (ITS) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM3007
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ViPrimePLUS Enterocytozoon bieneusi qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Enterocytozoon bieneusi qPCR Kit
