Grade
Molecular Biology Grade
Description
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA.
DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.
Specification
|
Appearance
|
White to off-white crystalline powder; a partially purified, lyophilized powder
|
|
Source
|
Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation
|
|
Unit Definition
|
One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.
|
Storage
Store at -20°C
|
Assay
|
Resuls
|
|
Activity
|
>500Kunitz U/mg
|
|
Loss on Drying
|
≤5.0%
|
|
Residue on Ignition
|
≤1.0%
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
PC0704-25mg
|
Deoxyribonuclease I (DNase I)
|
25mg
|
|
PC0704-50mg
|
Deoxyribonuclease I (DNase I)
|
50mg
|
|
PC0704-100mg
|
Deoxyribonuclease I (DNase I)
|
100mg
|
|
PC0704-1g
|
Deoxyribonuclease I (DNase I)
|
1g
|
Download
MSDS
Deoxyribonuclease I (DNase I)
