Grade
Ultra Pure Grade
Description
CHAPS is a standard reagent suitable for use as a solubilizing agent in isoelectric focusing applications is tested to meet low metal content specifications.
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent commonly used protein solubilization in isoelectric focusing and two-dimensional electrophoresis as well as used in breaking protein-protein interactions. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8M urea, 4% CHAPS, 50-100mM dithiothreitol (DTT), and 40mM Tris. Furthermore, its small micellar molecular weight (6150) and high critical micellar concentration (6-10mM) allow it to be removed from samples by dialysis.
The preparation of CHAPS is 50mg/ml. CHAPS is soluble in water.
Storage
Store at RT
Specification
|
Appearance
|
White to pale white powder
|
|
Mol. Formula
|
C32H58N2SO7
|
|
Mol. Weight
|
614.89
|
|
CAS RN
|
75621-03-3
|
|
Assay
|
Specifications
|
|
Purity
|
≥99.0%
|
|
Loss on drying
|
≤4.0%
|
|
TLC
|
One spot
|
|
Solubility (10% aq.)
|
Clear, colorless solution
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
PC0925-5g
|
3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)
|
5g
|
Download
MSDS
3-[(3-cholamidopropyl)dimethyl-ammonio]-1- propanesulfonate (CHAPS)
