Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activities enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.
Concentrations
100 - 500u/μl
Assay Conditions
50mM Tris- HCl (pH8.3), 6mM MgCl2, 10mM DTT, 0.4mM Poly(rA)•(dT)12-18, and 0.5mM [ 3H]dTTP in a reaction volume of 50μl./μl
Supplied With
10X Buffer M-MuLV RT
500mM Tris-HCl (pH 8.3 at 25˚C), 75mM KCl, 3mM MgCl2 and 10mM DTT. Store at -20˚C.
Storage Buffer
10mM K-Phosphate (pH 7.5), 0.1mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20˚C.
Thermal Inactivation
70˚C for 10 minutes
Unit Definition
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•oligo(dT).
Application
-
First strand cDNA synthesis.
-
DNA labeling.
-
RNA analysis by primer extension.
Quality Control
Purified free of contaminating endonucleases and exonucleases.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
ME2305
|
M-MuLV Reverse Transcriptase (RNase H¯)
|
10000u
|
|
ME2306
|
M-MuLV Reverse Transcriptase (RNase H¯)
|
50000u
|
Download
Manual
M-MuLV Reverse Transcriptase (RNase H¯)
Publication
This Product Has Been Used In:
Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(1), p. 29-35.
Noureini, S.K., Tanavar, F. (2015) Boldine, a natural aporphine alkaloid, inhibits telomerase at non-toxic concentrations. Chemico-Biological Interactions. 231. Pp27-34.
Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.
Ahmadi, E., Ahmadi, M., PourBahsh, S.A., Talebi, A. (2013). Detection and Differentiation of Newcastle Disease Virus Strains Affecting Commercial Poultry in Northwest of Iran using RT-PCR,International Journal of Veterinary Science, Vol 2, No. 4, 138-142.
Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).
