Description
The ViPrimePLUS Trypanosoma evansi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Trypanosoma evansi genome in clinical sample such as blood.
Trypanosoma evansi is the causative agent for trypanosomosis, also known as ‘surra’ disease in various wild and domesticated animals, particularly in Asia, Africa and Central and South America. Buffalo, cattle, camels and horses are the primary hosts for the pathogen. This protozoan is mainly transmitted via arthropods. Clinical signs of the disease include pyrexia, parasitemia, progressive anemia, loss of condition and lethargy. Edema at the lower body parts, haemorrhage of serous membrane and neurological signs may be observed in infected horses, while abortion in camels and buffalos. Trypanosomal DNA can be detected in animals with active infections (not more than 1-2days). Chronic infection may persist for years. The first case of human infection was reported in India, associating to 5-months febrile episodes. However, the pathogen is not known as zoonotic.
ViPrimePLUS Trypanosoma evansi qPCR Kit is intended for research use only.
Kit Component
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T. evansi Primers and Probe Mix (T. evansi PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Ruminant samples (e.g. blood)
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Gene Target
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Glycoprotein (VSG) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV6024
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ViPrimePLUS Trypanosoma evansi qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Trypanosoma evansi qPCR Kit
