Description
The ViPrimePLUS African Trypanosomiasis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of African Trypanosomiasis genomes in clinical samples (e.g. blood).
African Trypanosomiasis is known as the human sleeping sickness caused by Trypanosoma brucei gambiense (95%) and Trypanosoma brucei rhodesiense. This vector-borne parasitic disease is endemic in sub-Saharan Africa, where the tsetse fly vector is found. It is transmitted to human via the faeces of the fly near the bite wound. Another type of trypanosomiasis that occurs in Latin America is known as Chagas' disease which is caused by a different species of parasite. Currently the estimated actual cases are 30,000, which showed a great reduction compared to 50 years ago due to continuous prevention effort. The first stage haemolymphatic phase showed symptoms of fever, headaches and joint aches. The progression to the second stage entails the parasitic invasion of the blood-brain-barrier, thus infecting the central nervous system and causing neurological disorder. Without treatment, sleeping sickness is fatal.
ViPrimePLUS African Trypanosomiasis qPCR Kit is intended for research use only.
Kit Component
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A. Trypanosomiasis Primers and Probe Mix (A. Trypanosomiasis PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. blood)
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Gene Target
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18S ribosomal RNA
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM4001
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ViPrimePLUS African Trypanosomiasis qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS African Trypanosomiasis qPCR Kit
