Description
The ViPrimePLUS Newcastle Disease Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Newcastle Disease Virus genome in blood and oronasal swab samples.
Newcastle disease virus (NDV) is a single stranded RNA virus which infects birds, thus posing a major threat to the poultry industries and public health. Transmission is mainly via contact with infected birds secretions and faeces. There are 3 strains being recognized by its virulence; 1) velogenic (highly virulent), 2) mesogenic (intermediate virulence), and 3) lentogenic (mild virulence). Clinical signs of infection vary dependent on the pathotypes. The lentogenic strain may cause mild respiratory symptoms, while the mesogenic type may cause acute respiratory and neurologic diseases. Infection by velogenic strain may produce more severe neurological defects, damaged digestive system dropped in egg production, and eventually lead to death if left untreated. Exposure of infection to humans may not posed significant health hazards, but incidences of conjunctivitis and flu-like symptoms are reported.
ViPrimePLUS Newcastle Disease Virus RT-qPCR Kit is intended for research use only.
Kit Component
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NDV Primers and Probe Mix (NDV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Avian samples (blood, oronasal swab)
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Gene Target
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M gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV1021
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ViPrimePLUS Newcastle Disease Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Newcastle Disease Virus RT-qPCR Kit
