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产品介绍
品名	Polyclonal Antiserum to Human C8 Protein
货号	A309
规格	1.0 mL
形式	Whole Antiserum
防腐剂	≤ 0.1% Sodium Azide
物种交叉反应	Baboon, Dog, Rabbit, Guinea Pig, Cat, Hamster, Horse, Rat, Mouse
储存	2°C to 8°C  (≤ 30 days) For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.
使用说明	For Research Use Only. Not for use in diagnostic procedures
产品描述	Highly purified human C8 was isolated from normal serum and used to immunize goats. The anti-human C8 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C8 at varying concentrations.
背景	C8 is a glycoprotein which is present in normal human serum/plasma at approximately 50 µg/mL. C8 is unique in that it is made up of 3 non-identical subunits, α, β, and γ. The molecular weights of these subunits are 64 kD, 64 kD, and 22 kD, respectively. The α and γ chains are linked together by a disulfide bond, whereas the β chain is associated to the α-γ complex by a non-covalent bond. The main function of the C8 protein is to initiate the binding of C9 to form the Membrane Attack Complex (MAC).
应用	Applications of the C8 polyclonal antisera have been evalsuated by various research facilities, and include Immunofluorescence1 and ELISA. 2
参考文献	1、Meri, S. et al. “Activation of the Alternative Pathway of Complement by Monoclonal Light Chains in Membranoproliferative Glomerulonephritis.” The Journal of Experimental Medicine (1992): 939-950.  2、Abdulla, Salima Abubaker. Developmental Innate Immunoinsufficiency: Comparison of Term Neonatal Neutrophil Proteinases and Complement Component Levels Relative to Adults. Diss. Cardiff University School of Medicine, United Kingdom, 2012. Web